One-Step Blue® Protein Gel Stain
One-Step Blue® Protein Gel Stain
One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein gel staining in a single step without fixation or washing. One-Step Blue® is meant as a replacement for Coomassie stain.
One-Step Stains Features
Fast, simple staining
No fixing, microwaving, or washing
Aqueous-based and non-toxic for drain disposal
Please contact use for samples.
PRODUCT DESCRIPTION
One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence. In addition to rapid results and simple staining, One-Step Blue® offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid. For more information, download the One-Step Stains Safety Report.
One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing.
Biotium also offers One-Step Lumitein™ Protein Gel Stain, a rapid, easy-to-use, non-toxic red fluorescent protein gel stain for detection using a UV transilluminator or laser gel scanner, that costs significantly less than other fluorescent protein gels stains.